Protocols in Root-Microbe Interactions

Authors

Anjali S. Iyer-Pascuzzi, Purdue University

Files

Page Count

130

Language

English

Description

Protocols in Root-Microbe Interactions describes laboratory protocols that have been developed and optimized for scientists working in root-microbe interactions, specifically for those wanting to quantify immune responses in plant roots. The experiments are focused on cellular to whole-root scale and include molecular biology, biochemistry, and histology techniques. Written in an easy-to-understand fashion that is suitable for both early career and experienced scientists, the protocols were optimized for tomato roots but can easily be adapted to roots of other plant species, or if needed, to leaves. The book includes information on quantifying pattern-triggered immune (PTI) responses in plant roots and covers reactive oxygen species (ROS) burst, mitogen-activated protein kinase (MAPK) phosphorylation, root growth inhibition, and qualitative callose deposition. Other topics examined include transient expression of bacterial proteins in leaves and root sectioning (from a microtome and vibratome) and histology assays for both light microscopy and scanning electron microscopy. A variety of other protocols explored relate to the isolation of bacteria from the root endosphere and rhizosphere, and the extraction of DNA from root-associated bacteria. Protocols in Root-Microbe Interactions serves as an invaluable resource for those working in the root-microbe field and can be optimized for other laboratories’ needs.

ISBN

9781626712829

Publication Date

Winter 2-15-2026

Publisher

Purdue University Press

City

West Lafayette

Keywords

plant immunity, bacteria, fungi, viruses, plant defense, pattern-recognition receptors, antimicrobial compounds, intracellular immune receptors, salicylic acid, jasmonic acid, ethylene, disease resistance, chemiluminescent assay, media preparation, seed sterilization, luminescence attenuation, elicitor mixes, western blot, protein extraction, Bradford assay, Laemmli buffer, PonceauS stain, root imaging, histology assays, ethanol, root colonization, endophytes, auxin production, siderophore production, phosphorus solubilization

Disciplines

Life Sciences

Comments

Open access publication of this title is supported by Purdue University Libraries and School of Information Studies.

Recommended Citation

Iyer-Pascuzzi, Anjali S., "Protocols in Root-Microbe Interactions" (2026). Purdue University Press Books. 111.
https://docs.lib.purdue.edu/purduepress_ebooks/111