Date of Award
Fall 2013
Degree Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Chemistry
First Advisor
Scott A. McLuckey
Committee Chair
Scott A. McLuckey
Committee Member 1
Mary J. Wirth
Committee Member 2
Mingji Dai
Committee Member 3
David H. Thompson
Abstract
Gas-phase ion/ion chemistry involves the interaction of oppositely charged ions inside of the mass spectrometer. During this gas-phase chemistry, particle transfer (i.e., proton and electron) or synthesis can occur at rapid reaction rates. Particle transfer represents a mature area of ion/ion chemistry, while selective covalent modification represents a fairly new area of gas-phase chemistry. Gas-phase covalent chemistry is based on traditional solution phase organic chemistry.
The work demonstrated in this dissertation greatly involves gas-phase covalent and non-covalent Schiff base chemistry on peptide and protein ions. The reagent dianion, 4-formyl 1,3-benzene disulfonic acid, has been used to covalently modify unprotonated primary amines present in peptide and protein ions. In addition, strong non-covalent interactions have also been observed with arginine-containing peptides ions. Studies of their dissociation behavior as well as the nature of their interaction (i.e., covalent versus non-covalent) have been investigated. Application of this Schiff base ion/ion chemistry has been demonstrated on matrix assisted laser desorption/ionization (MALDI)-derived peptide ions. Such Schiff base ion/ion chemistry, whether electrospray or MALDI-derived, can produce complementary or even an increase in structural information. Multiple covalent modifications within one ion/ion encounter have been demonstrated on peptide and protein cations via Schiff base cluster anions. This dissertation also highlights the gas-phase transformation of phosphatidylcholine cations into demethylated phosphatidylcholine anions, which provides an increase in structural information upon activation. As a whole, gas-phase covalent and non-covalent ion/ion chemistry represents a promising new area for identifying and characterizing biological analytes.
Recommended Citation
Stutzman, John Robert, "Gas-Phase Covalent And Non-Covalent Ion/ion Chemistry Of Biological Macromolecules" (2013). Open Access Dissertations. 46.
https://docs.lib.purdue.edu/open_access_dissertations/46
