Date of Award

January 2016

Degree Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Agricultural and Biological Engineering

First Advisor

JOSEPH MK IRUDAYARAJ

Committee Member 1

PAUL J ROBINSON

Committee Member 2

MENG DENG

Committee Member 3

JENNA RICKUS

Abstract

Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM technique combined with Time Correlated Single Photon Counting (TCPSC) Technology was used to develop several novel biosensing approaches. Using linear or nonlinear excitation source, TCSPC-FLIM enabled real time monitoring of cellular metabolite profiles, as well as longitudinal observation of post-translational enzyme activity such as kinase phosphorylation and acetyltransferase acetylation. This technique will not only provide high spatiotemporal resolution of sensing output but also for the first time, realize real-time biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish.

Download

Share

COinS