An Investigation of a Cluster S Mycobacteriophage Genome, Corazon, Genes 4-16: Location and Function
Select the category the research project fits.
Life Sciences
Is this submission part of ICaP/PW (Introductory Composition at Purdue/Professional Writing)?
No
Abstract
Mycobacteriophages are a type of virus particle that specifically attack mycobacteria. This attribute can be exploited to fight antibiotic resistance mycobacteria. As of February 2019, only 14 Cluster S types (a specific group of mycobacteriophage) have been completely sequenced and published in the Actinobacteriophage Database. The purpose of this investigation is to establish the presence, location, and function of genes within the genome of a novel bacteriophage, Corazon and add to this database. Corazon, isolated from a soil sample collected in Lafayette, Indiana, is a member of Cluster S and belongs to the Siphoviridae morphotype. It has 109 genes and a 64kbp genome size. The genome was analyzed with the program DNA Master and a variety of sources such as NCBI BLAST, HHPred, and Phamerator to determine the location and function of genes within the auto-annotated range of genes 4-16. Within the investigated range, only one gene was assigned a function (MazG-like nucleotide pyrophosphohydrolase, which interacts with an essential GTPase in bacteria). The other 13 genes were annotated as genes with no known function. The investigation of significant gaps in the genome resulted in an additional gene (14.5) being added. Contributing to the global understanding of bacteriophages is of interest since the phage-bacteria model has expanded scientists’ capabilities of studying evolution and exploring novel medical applications. Publishing these annotations will allow generations of researchers to compare their results to this member of Cluster S and potentially identify a new candidate for phage-mediated transduction, phage therapy, or other application.
Recommended Citation
Mehling, Marina; Cline, Eli; Wilson, Remington; and Pineau, McKenna, "An Investigation of a Cluster S Mycobacteriophage Genome, Corazon, Genes 4-16: Location and Function" (2019). Purdue Undergraduate Research Conference. 36.
https://docs.lib.purdue.edu/purc/2019/Posters/36
An Investigation of a Cluster S Mycobacteriophage Genome, Corazon, Genes 4-16: Location and Function
Mycobacteriophages are a type of virus particle that specifically attack mycobacteria. This attribute can be exploited to fight antibiotic resistance mycobacteria. As of February 2019, only 14 Cluster S types (a specific group of mycobacteriophage) have been completely sequenced and published in the Actinobacteriophage Database. The purpose of this investigation is to establish the presence, location, and function of genes within the genome of a novel bacteriophage, Corazon and add to this database. Corazon, isolated from a soil sample collected in Lafayette, Indiana, is a member of Cluster S and belongs to the Siphoviridae morphotype. It has 109 genes and a 64kbp genome size. The genome was analyzed with the program DNA Master and a variety of sources such as NCBI BLAST, HHPred, and Phamerator to determine the location and function of genes within the auto-annotated range of genes 4-16. Within the investigated range, only one gene was assigned a function (MazG-like nucleotide pyrophosphohydrolase, which interacts with an essential GTPase in bacteria). The other 13 genes were annotated as genes with no known function. The investigation of significant gaps in the genome resulted in an additional gene (14.5) being added. Contributing to the global understanding of bacteriophages is of interest since the phage-bacteria model has expanded scientists’ capabilities of studying evolution and exploring novel medical applications. Publishing these annotations will allow generations of researchers to compare their results to this member of Cluster S and potentially identify a new candidate for phage-mediated transduction, phage therapy, or other application.