Title

Patterned protein microarrays for bacterial detection

Published in:

Langmuir 19,2 (2003) 436-439;

Abstract

Patterned microarrays of antibodies were fabricated and tested for their ability to bind targeted bacteria. These arrays were used in a series of bacterial immunoassays to detect E. coli O157:147 and Renibacterium salmoninarum (RS). Microarrays were fabricated using microcontact printing (muCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that targeted bacteria had a higher binding selectivity to complementary antibody patterns than to unfunctionalized regions of the substrate. Additional studies indicated a significant reduction in binding of bacteria when the microarrays were exposed to nonspecific bacteria. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.

Keywords

self-assembled monolayers;; quartz-crystal microbalance;; cell detection;; gold;; fabrication;; diffraction;; microscopy;; salmonella;; features;; layers

Date of this Version

January 2003

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