Patterned protein microarrays for bacterial detection
Abstract
Patterned microarrays of antibodies were fabricated and tested for their ability to bind targeted bacteria. These arrays were used in a series of bacterial immunoassays to detect E. coli O157:147 and Renibacterium salmoninarum (RS). Microarrays were fabricated using microcontact printing (muCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that targeted bacteria had a higher binding selectivity to complementary antibody patterns than to unfunctionalized regions of the substrate. Additional studies indicated a significant reduction in binding of bacteria when the microarrays were exposed to nonspecific bacteria. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.
Published in:
Langmuir 19,2 (2003) 436-439;
Link to original published article:
http://dx.doi.org/10.1021/la026365+
Keywords
self-assembled monolayers;; quartz-crystal microbalance;; cell detection;; gold;; fabrication;; diffraction;; microscopy;; salmonella;; features;; layers
Date of Version
January 2003
Recommended Citation
Howell, S. W.; Inerowicz, H. D.; Regnier, F. E.; and Reifenberger, R., "Patterned protein microarrays for bacterial detection" (2003). Department of Physics and Astronomy Faculty Publications. Paper 432.
https://docs.lib.purdue.edu/physics_articles/432