Date of Award
Spring 2014
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Mary J. Wirth
Committee Member 1
Chengde Mao
Committee Member 2
Hilkka I. Kenttamaa
Abstract
PSA has been used as a biomarker for prostate cancer for a long time. To characterize different glycoforms of PSA using techniques like UHPLC and cIEF, concentration and purification of PSA from complex samples becomes necessary. Our group has developed submicron silica spheres based affinity beads to extract PSA through immunoprecipitation. This study focuses on measuring the bead performance when antibody type/amount was varied and PSA concentration was lowered for clinical use. To better satisfy the requirement of large-scale extraction, several attempts were made to increase the bead capacity. The results will be presented and future directions of the project will be discussed.
Recommended Citation
Wang, Yao, "Protein Affinity Extraction Of Prostate Specific Antigen (PSA) Using Submicron Spheres" (2014). Open Access Theses. 280.
https://docs.lib.purdue.edu/open_access_theses/280