Date of Award
Fall 2013
Degree Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Biological Science
First Advisor
Jeffrey T. Bolin
Second Advisor
Dinesh A. Yernool
Committee Chair
Jeffrey T. Bolin
Committee Member 1
Dinesh A. Yernool
Committee Member 2
Eric L. Baker
Committee Member 3
William A. Cramer
Abstract
To better understand a class of transporters known as Calcium/Cation Antiporters (CaCAs), the bacterial calcium/proton antiporter CAXCK31 was purified and characterized. New methods were developed for its heterologous overexpression and purification. These methods help to define stress responses to toxic membrane overproduction in E. coli and may be broadly applicable to studies of membrane proteins. The results from a variety of biochemical and biophysical experiments demonstrated that CAXCK31 exists as a dimer in the membrane and can be purified in the dimeric state. The methods used include chemical cross-linking, FRET, and SEC-MALS. In addition, various transport properties of CAXCK31, including substrate selectivity, pH dependence, and transport rates, have been characterized for the first time.
Recommended Citation
Ridilla, Marc Robert, "Characterization of CAXCK31, a Bacterial Calcium/Proton Antiporter" (2013). Open Access Dissertations. 76.
https://docs.lib.purdue.edu/open_access_dissertations/76
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