Date of Award

January 2015

Degree Type


Degree Name

Doctor of Philosophy (PhD)



First Advisor

Elizabeth J Tran

Committee Member 1

Barbara L Golden

Committee Member 2

Mark C Hall

Committee Member 3

Robert L Geahlen


In eukaryotes, there are highly coupled mechanisms that require RNA-binding proteins to facilitate gene expression. Proper RNA structure and ribonucleoprotein (RNP) complex formation are critical for gene expression. DEAD-box proteins are the largest class of RNA helicases that play fundamental roles in RNA and RNP structure remodeling. However, the precise biological role of the vast majority of the ~ 40 members in this family has not been completely described. Therefore, my research focused on characterizing the role of the DEAD-box RNA helicase Dbp2 during gene expression in S. cerevisiae.