Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications

Author

• Yu-Ho Won, Purdue University - Main CampusFollow
• Ho Seong Jang, Purdue University - Main CampusFollow
• Seung Min Kim, Purdue University - Main CampusFollow
• E A. Stach, Birck Nanotechnology Center and School of Materials Engineering, Purdue UniversityFollow
• Mallikarjunarao Ganesana, Clarkson University
• Silvana Andreescu, Clarkson University
• L Stanciu, Birck Nanotechnology Center and School of Materials Engineering, Purdue UniversityFollow

Abstract

In this work, a novel avenue to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities to be used in the design of highly stable, magnetically separable enzyme-based systems was explored. As a model system, immobilization of acetylcholinesterase (AChE) was investigated using biomagnetic glasses composed of a magnetic core with a size tunable porous silica shell. The efficiency of the immobilization was determined by analyzing the biosensing capability of these biomagnetic glasses for the detection of the organophosphorous pesticide paraoxon. Screen printed electrodes with the AChE-biomagnetic glasses showed higher current response and stability than for the free enzyme. The detection limit of the paraoxon biosensor was in the nanomolar range.

Keywords

SCREEN-PRINTED ELECTRODES; CARBON-PASTE ELECTRODE; SOL-GEL COMPOSITE; MAGNETIC NANOPARTICLES; ELECTROCHEMICAL DETECTION; DNA HYBRIDIZATION; QUANTUM DOTS; IMMOBILIZATION; ENZYME; ACETYLCHOLINESTERASE

Date of this Version

3-16-2010

Recommended Citation

Won, Yu-Ho; Jang, Ho Seong; Kim, Seung Min; Stach, E A.; Ganesana, Mallikarjunarao; Andreescu, Silvana; and Stanciu, L, "Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications" (2010). Birck and NCN Publications. Paper 621.
https://docs.lib.purdue.edu/nanopub/621