Abstract
We report a simple and robust magnetomechanical system for direct visual observation of the DNA packaging behavior of the bacteriophage phi29 in real time. The system comprises a micron-sized magnetic bead attached to the free end of the viral DNA, a magnet and a bright-field microscope. We show that the phi29 DNA packaging activity can be observed and dynamically analyzed at the single molecular level in bright field with a relatively simple system. With this system we also visually demonstrate the phi29 motor transporting a cargo 10 000 times the viral size.
Keywords
BACILLUS-SUBTILIS; PORTAL MOTOR; PROTEIN GP16; BACTERIOPHAGE PHI-29; RNA NANOTECHNOLOGY; BACTERIAL-VIRUS; INTERNAL FORCE; GENE DELIVERY; PHAGE PHI-29; PRNA
Citation
APPLIED PHYSICS LETTERS 93, 153902 (2008)
Date of this Version
10-13-2008
Recommended Citation
Chang, Chun-Li; Zhang, Hui; Shu, Dan; Guo, Peixuan; and Savran, Cagri A., "Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system" (2008). Birck and NCN Publications. Paper 329.
https://docs.lib.purdue.edu/nanopub/329