Electrical characterization of DNA molecules in solution using impedance measurements

Author

• Yi-Shao Liu, ECE, Purdue UniversityFollow
• Padmapriya Banada, Purdue University
• Shantanu Bhattacharya, Purdue University
• Arun K. Bhunia, Department of Food Science, Purdue UniversityFollow
• Rashid Bashir, Birck Nanotechnology Center and Bindley Bioscience Center, Purdue UniversityFollow

Abstract

We report on impedance measurements of fluids to examine the impact of the length and concentration of free-floating double-stranded DNA molecules. The impedance versus frequency characteristics were fitted to an equivalent circuit model including solution dielectric capacitance and conductance. The extraction of these parameters can be used to detect the presence of DNA molecules in the nanomolar range for a 400 bp long molecule. Our results show that the extracted dielectric capacitance and conductance increase with molecule length and concentration owing to a corresponding increase in number of molecule dipoles and counterions in solution.

Comments

This is the publisher PDF of Liu, YS; Banada, PP; Bhattacharya, S; Bhunia AK; and Bashir, R. "Electrical characterization of DNA molecules in solution using impedance measurements." Applied Physics Letters 92 (2008). Copyright American Institute of Physics, it is available at http://dx.doi.org/10.1063/1.2908203.

DOI

10.1063/1.2908203

Citation

APPLIED PHYSICS LETTERS 92, 143902

Date of this Version

4-7-2008

Recommended Citation

Liu, Yi-Shao; Banada, Padmapriya; Bhattacharya, Shantanu; Bhunia, Arun K.; and Bashir, Rashid, "Electrical characterization of DNA molecules in solution using impedance measurements" (2008). Birck and NCN Publications. Paper 173.
http://dx.doi.org/10.1063/1.2908203