Disease-on-a-chip: mimicry of tumor growth in mammary ducts

Author

• Pierre-Alexandre Vidi, Purdue UniversityFollow
• Teimour Maleki, Purdue University, Birck Nanotechnology CenterFollow
• Manuel Ochoa, Purdue University, Birck Nanotechnology CenterFollow
• Lei Wang, Purdue University
• Sara M. Clark, Purdue University
• James F. Leary, Purdue University, Birck Nanotechnology CenterFollow
• Sophie A. Lelievre, Purdue UniversityFollow

Abstract

We present a disease-on-a-chip model in which cancer grows within phenotypically normal breast luminal epithelium on semicircular acrylic support mimicking portions of mammary ducts. The cells from tumor nodules developing within these hemichannels are morphologically distinct from their counterparts cultured on flat surfaces. Moreover, tumor nodules cocultured with the luminal epithelium in hemichannels display a different anticancer drug sensitivity compared to nodules cocultured with the luminal epithelium on a flat surface and to monocultures of tumor nodules. The mimicry of tumor development within the epithelial environment of mammary ducts provides a framework for the design and test of anticancer therapies.

Keywords

BREAST EPITHELIAL-CELLS, MAMMALIAN-CELLS, IN-VITRO, CANCER, COCULTURE, GRADE

DOI

10.1039/c3lc50819f

Date of this Version

2014

Recommended Citation

Vidi, Pierre-Alexandre; Maleki, Teimour; Ochoa, Manuel; Wang, Lei; Clark, Sara M.; Leary, James F.; and Lelievre, Sophie A., "Disease-on-a-chip: mimicry of tumor growth in mammary ducts" (2014). Birck and NCN Publications. Paper 1534.
http://dx.doi.org/10.1039/c3lc50819f