Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices
Abstract
We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally enhanced pyrophosphate (PPi) signal generation and sensors with immobilized chelators for capture and surface-sensitive detection of diffusible reaction by-products. While detecting arrays of enzymatic base incorporation reactions is typically accomplished using optical fluorescence or chemiluminescence techniques, our results suggest that it is possible to develop scalable and portable PPi-specific sensors and platforms for broad biomedical applications such as DNA sequencing and microbe detection using surface-sensitive electrical readout techniques.
Keywords
EFFECT TRANSISTORS; SENSOR ARRAYS; AMPLIFICATION; BIOSENSORS; BIORECOGNITION; HYBRIDIZATION; SPECTROSCOPY; DIAGNOSTICS; MOLECULES; EMULSION
DOI
10.1039/c2an15930a
Citation
Analyst, 2012,137, 1351-1362 DOI: 10.1039/C2AN15930A
Date of this Version
12-30-2011
Recommended Citation
Credo, Grace M.; Su, Xing; Wu, Kai; Elibol, Oguz; Liu, David J.; Reddy, Bobby Jr.; Tsai, Ta-Wei; Dorvel, Brian R.; Daniels, Jonathan S.; Bashir, Rashid; and Varma, Madoo, "Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices" (2011). Birck and NCN Publications. Paper 1305.
http://dx.doi.org/10.1039/c2an15930a