Raman Chemical Imaging of Chromate Reduction Sites in a Single Bacterium Using Intracellularly Grown Gold Nanoislands
Abstract
Imaging live molecular events within micro-organisms at single-cell resolution would deliver valuable mechanistic information much needed in understanding key biological processes. We present a surface-enhanced Raman (SERS) chemical imaging strategy as a first step toward exploring the intracellular bioreduction pockets of toxic chromate in Shewanella. In order to achieve this, we take advantage of an innate reductive mechanism in bacteria of reducing gold ions Into intracellular gold nanoislands, which provide the necessary enhancement for SERS imaging. We show that SERS has the sensitivity and selectivity not only to identify but also to differentiate between the two stable valence forms of chromate in cells. The imaging platform was used to understand intracellular metal reduction activities In a ubiquitous metal-reducing organism, Shewanella oneidensis MR-1, by mapping chromate reduction.
Keywords
surface-enhanced Raman spectroscopy; Shewanella oneidensis MR-1; single-cell Raman imaging; hexavalent chromate; bioremediation; SURFACE-ENHANCED RAMAN; SHEWANELLA-ONEIDENSIS; GENOME SEQUENCE; METAL-SURFACES; SPECTROSCOPY; SCATTERING; CELLS; NANOPARTICLES; SERS; CHROMIUM
DOI
10.1021/nn201105r
Citation
Raman Chemical Imaging of Chromate Reduction Sites in a Single Bacterium Using Intracellularly Grown Gold Nanoislands Sandeep P. Ravindranath, Kristene L. Henne, Dorothea K. Thompson, and Joseph Irudayaraj ACS Nano 2011 5 (6), 4729-4736 DOI: 10.1021/nn201105r
Date of this Version
6-2011
Recommended Citation
Ravindranath, Sandeep; Henne, Kristene L.; Thompson, Dorothea; and Irudayaraj, Joseph, "Raman Chemical Imaging of Chromate Reduction Sites in a Single Bacterium Using Intracellularly Grown Gold Nanoislands" (2011). Birck and NCN Publications. Paper 1004.
http://dx.doi.org/10.1021/nn201105r