Recommended Citation
Derré, M. G., Steinbach, S. M., Hendrix, G. K., Stanton, M., & Adams, L. G. (2023). Iodinated non-ionic contrast medium does not inhibit Escherichia coli growth in ex vivo inoculated canine urine. American Journal of Veterinary Research, 84(7), ajvr.23.03.0045. Retrieved Feb 16, 2024, from https://doi.org/10.2460/ajvr.23.03.0045
DOI
https://doi.org/10.2460/ajvr.23.03.0045
Date of this Version
5-14-2023
Abstract
OBJECTIVE
To investigate the effect of iohexol on standardized quantitative urine culture results in dogs. The authors hypothesized that the presence of iohexol in inoculated urine samples would result in lower bacterial concentrations (CFU/mL) and, therefore, decrease culture sensitivity.
SAMPLE
Urine samples were aseptically collected during cystoscopy from a single client-owned dog untreated with antimicrobials.
PROCEDURES
An experimental controlled study. The urine sample was divided into 38 aliquots (0.5 mL each) that were used as negative controls or inoculated with an equal amount of Escherichia coli (105 CFU/mL). Different volumes (0.1 and 0.5 mL) of contrast or saline were added to the aliquots and quantitative culture results were compared. Two different incubation times between the preparation of aliquots and culture were evaluated (15 minutes and 24 hours).
RESULTS
All aliquots from samples inoculated with E. coli (positive controls and iohexol-group) had the same reported quantitative result (104 CFU/mL). No growth was reported for the negative controls. Iohexol did not show any anti-E. coli properties in canine urine cultures for dilutions up to 1:2 contrast:urine and concentrations up to 120 mgI/mL. No difference was reported when iohexol was incubated with inoculated urine for 15 minutes or 24 hours.
CLINICAL RELEVANCE
Based on the experimental in vitro conditions described, administration of iohexol before the collection of urine during urologic procedures does not negatively impact the isolation and growth of E. coli.
Comments
This is the published version of the Derré, M. G., Steinbach, S. M., Hendrix, G. K., Stanton, M., & Adams, L. G. (2023). Iodinated non-ionic contrast medium does not inhibit Escherichia coli growth in ex vivo inoculated canine urine. American Journal of Veterinary Research, 84(7), ajvr.23.03.0045. Retrieved Feb 16, 2024, from https://doi.org/10.2460/ajvr.23.03.0045