Recommended Citation
Park, Seungbin; Lipton, Megan; Sun, Yujiao J.; and Dadarlat, Maria, "Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging" (2024). Purdue University Libraries Open Access Publishing Fund. Paper 213.
http://dx.doi.org/10.1016/j.xpro.2024.103027
DOI
10.1016/j.xpro.2024.103027
Date of this Version
6-21-2024
Abstract
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window.We additionally describe the procedures for viral injections and awake head-fixed recordings.
Comments
This is the publisher PDF of Park, S; Lipton, M; Sun, YJ; and Dadarlat, M. (2024) Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. STAR Protocols, 5(2): 103027. This article is distributed under a CC-BY-NC-ND license, and is available at DOI: 10.1016/j.xpro.2024.103027.