Preparation of alginate microparticles by emulsification for oral vaccine delivery
Abstract
The traditional method of vaccine delivery is by parenteral administration. However, delivery of vaccine by the oral route offers great potential in producing systemic and local immune responses at various mucosal surfaces not only in the GI-tract where infection begins but also in the lung, salivary glands, and genitourinary region. Preparation of alginate microparticles by an emulsification method was investigated. Formulation effects of the type and concentration of the surfactant and cation on the particle size and size distribution of the alginate microparticles was also investigated. The results obtained indicated that surfactant HLB, structure and concentration as well as the concentration of the cation and alginate played an important role in the formation of alginate microparticles within the 1-10 $\mu$m size range. Microparticles in the size range of 2-5 $\mu$m were obtained using high concentration of surfactant sorbitan trioleate at a HLB of 1.8, high concentration of zinc chloride, and a sodium alginate concentration of 1.2% (w/v).
Degree
Ph.D.
Advisors
Park, Purdue University.
Subject Area
Pharmacology|Pharmaceuticals
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