The synthesis and characterization of polyacrylate anion-exchange stationary phases for protein separations in liquid chromatography
Abstract
A new type of anion-exchange stationary phase was synthesized utilizing a polyacrylate polymer grafted to Licrospher 1000 diol silica particles which was used for protein separations in liquid chromatography. These materials exhibited superior chromatographic abilities, as well as producing static binding capacities which were 3-5 times the values obtained with conventional ion-exchange resins. Chapter one of this thesis will present a literature overview of the types of packing materials used for liquid chromatographic protein separations over the last three and a half decades, and discuss advantages and disadvantages of each type of packing material. Chapter 2 will present the synthetic schemes, as well as methods for characterizing these polyacrylate anion-exchange sorbents, such as elemental analysis and protein binding capacity results, as well as displaying some typical chromatograms that can be obtained with them. Discussion will also be spent upon the type of organic mechanism that these materials undergo during their polymerization reactions, utilizing methyl vinyl ketone and picric acid. Chapter 3 will present the results obtained from a thorough and systematic Z number study performed with these polyacrylate anion-exchange resins, and study trends observed associated with the nature of the stationary phase ligands produced. Chapter 4 of this thesis will discuss the thermodynamic parameters obtained with these ion-exchange sorbents, again studying trends observed based upon the nature of the polymerization products synthesized. Chapter 5 will portray the polymerization products produced from utilizing N- (Tris(hydroxymethyl)methyl) acrylamide as an initial starting monomer in the synthesis of anion-exchange resins, which should yield higher binding capacities than their diol silica counterparts, as well as again presenting the typical protein chromatograms that can be collected with columns manufactured from these materials.
Degree
Ph.D.
Advisors
Regnier, Purdue University.
Subject Area
Analytical chemistry|Biochemistry
Off-Campus Purdue Users:
To access this dissertation, please log in to our
proxy server.