Digital data analysis, chemiluminescent detection, and enzymatic chemical amplification for capillary electrophoresis
Abstract
Three techniques were investigated as methods to lower detection limits of capillary electrophoresis. These were digital data analysis, chemiluminescent detection, and enzymatic chemical amplification. Digital methods included ensemble averaging, digital filtering, and Fourier transform filtering. Instrumentation for chemiluminescent detection in capillary electrophoresis required the construct of novel capillary arrangements, light detection, and data collection. The chemiluminescent reactions of luminol and firefly luciferase were investigated for post-column detection. The analytes hydrogen peroxide, adenosine triphosphate, proline, alanine, and adenosine diphosphate were detected. Enzymatic chemical amplification further lowered the detection limits of chemiluminescent detection. The enzymes horseradish peroxidase, alkaline phosphatase, urate oxidase, amino acid oxidase, galactose oxidase, glucose oxidase, and catalase were detected. Chemiluminescent sensors were demonstrated for hydrogen peroxide, horseradish peroxidase, and proline. Multichannel chemiluminescent detection was performed with a charge-coupled device camera.
Degree
Ph.D.
Advisors
Regnier, Purdue University.
Subject Area
Analytical chemistry|Biochemistry
Off-Campus Purdue Users:
To access this dissertation, please log in to our
proxy server.