Capillary ultrafiltration as an in vivo sampling technique for the investigation of endogenous species
Abstract
Capillary ultrafiltration probes consisting of three loops of hollow dialysis fibers have been used in this research to acquire physiological samples while causing only a minimum perturbation to the system being studied so that the collected sample accurately represents the site under investigation. Since the technique actively pulls fluid into the probe, the process is not dependent on diffusion and the collected sample should match the physiological fluid quite closely. Ultrafiltration probes were used to monitor amino acid concentrations in saliva and compare these values to blood concentrations. The probes were also implanted in the subcutaneous space of rats. It was determined that amino acid concentrations in this fluid are fairly well regulated and consistent between multiple animals. Implantation of the probes in horses enabled the study of sodium, potassium, calcium and amino acids in the subcutaneous and muscle tissue as compared to plasma. Sodium and potassium have similar concentrations among the compartments investigated unlike calcium and amino acids. Distribution kinetics were also studied.
Degree
Ph.D.
Advisors
Kissinger, Purdue University.
Subject Area
Analytical chemistry|Biochemistry|Anatomy & physiology|Animals
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