DNA fingerprinting and population genetics studies in the red flour beetle
Abstract
The main purpose of the study was to identify a probe which could facilitate an integration of molecular markers into the quantitative and population genetics of Tribolium. It is shown that it is possible to produce DNA banding patterns in the red flour beetle resembling DNA fingerprints in other species using as a probe a mid-repetitive DNA sequence of Tribolium's genome. The variability of these banding patterns was found to be comparable with that detected by DNA fingerprinting in domestic animals (Georges et al., 1988). It was also shown that these molecular polymorphisms provide stably inherited genetic markers that can be reliably used for studying the segregation of multiple heterozygous DNA fragments. The relationship between these polymorphisms and the degree of inbreeding was also established. Finally, results from a linkage analysis between the DNA fragments and large pupal weight in an F2 population and in 5 backcrossed families reflected different fragments associated with large pupal weight.
Degree
Ph.D.
Advisors
Stuart, Purdue University.
Subject Area
Genetics|Molecular biology|Entomology
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