Surface modification of poly(styrene-divinylbenzene) supports for protein separations
Abstract
The purpose of this research was to develop surface coating chemistries on poly(styrene-divinylbenzene) packings for protein separations. This is achieved by coating a thin layer of polymer onto PS-DVB packing materials to mask the hydrophobic surface of the support and to produce stationary phases with a hair like boundary referred to as "fimbriate" stationary phases. Two different types of coatings used to generate fimbriate phases are examined in this dissertation. The first is based on poly(glycidyl methacrylate). This polymer can be synthesized through a ring opening polymerization reaction. After coating the polymer onto PS-DVB surface, a free radical reaction can crosslink the coating, producing a variety of fimbriate phases and bonding the coating to the PS-DVB support simultaneously. The second involves modifying polyvinyl alcohol (PVA) to create double bonds. Crosslinking and bonding the coating to PS-DVB are accomplished in a free radical reaction. Derivatizations to produce fimbriate phases can be achieved in separate steps. The two coatings showed excellent performance in protein separations and each has its own advantages. The polymers and coated packings were characterized by chromatographic and spectroscopic methods. Syntheses and performance of alkylated PS-DVB packings are also described in this thesis.
Degree
Ph.D.
Advisors
Regnier, Purdue University.
Subject Area
Organic chemistry|Polymers|Analytical chemistry
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