Immunohistochemical and DNA ploidy analysis of canine transitional cell carcinoma of urinary bladder
Abstract
In the dog, transitional cell carcinoma of the urinary bladder has not been well characterized beyond the light microscopic level. The studies reported in this thesis were undertaken to further characterize canine transitional cell carcinoma through the use of cytometric and immunohistochemical techniques. The cytometric techniques of flow cytometry and image analysis were used to measure the DNA ploidy while an avidin-biotin-complex immunoperoxidase method, using the monoclonal antibody B72.3, was used to evaluate for the expression of the tumor-associated glycoprotein (TAG)-72. The DNA ploidy of canine transitional cell carcinoma was successfully measured by flow cytometry and image analysis. Seventy-nine percent of the neoplasms were aneuploid and 21% were diploid. Even though a majority of the neoplasms were aneuploid, DNA ploidy did not appear to provide any prognostic information because no significant correlation was observed between the DNA ploidy and various types of clinicopathologic features. The finding that a majority of transitional cell carcinoma samples stained positive for MAb B72.3 is suggestive that an antigen similar to TAG-72 was expressed in this neoplasm. The presence of similar staining patterns between MAb B72.3 and two other anti-TAG-72 antibodies (MAbs CC49 and CC83) was additional support for the expression of TAG-72 in canine transitional cell carcinomas. The preferential binding of MAb B72.3 for a tumor-associated antigen in neoplastic epithelial cells was further substantiated by the finding that this antibody stained other types of canine carcinomas, including adenocarcinomas from the gastrointestinal tract, pancreas, lung, mammary gland, nose, and prostate gland. The finding that MAb B72.3 did not stain any of the nonepithelial neoplasms and most types of normal tissues was additional support for the selective immunoreactivity of this antibody for canine neoplastic epithelial cells. Even though the presence of DNA aneuploidy and staining of MAb B72.3 appear to be features of canine transitional cell carcinoma, no correlation was observed between them. In conclusion, the techniques of immunohistochemistry and cytometry have helped characterize canine transitional cell carcinoma beyond the light microscopic level.
Degree
Ph.D.
Advisors
DeNicola, Purdue University.
Subject Area
Veterinary services|Animal diseases
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