Chromatographic competitive binding immunoassays using protein A and G affinity chromatography
Abstract
The combination of classical competitive binding immunoassays and high-performance affinity chromatography was used to develop rapid immunoassays for use in biotechnology, clinical, and pharmaceutical applications. Competitive binding immunoassays were carried out on protein A and G columns in both kinetic (immunological reaction on-column) and equilibrium (immunological reaction before injection) modes. While both modes were shown to be accurate and rapid quantitative tools, the equilibrium modes were faster and more sensitive. Simultaneous addition equilibrium mode assays could be carried out in as fast as 10 seconds, and the same mode had a detection limit of $3.8\times 10\sp{-9}$ M albumin, or 760 femtomoles, using laser-induced fluorescence.
Degree
Ph.D.
Advisors
Regnier, Purdue University.
Subject Area
Analytical chemistry
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