An investigation of the photochemical and non-photochemical interactions of rhodium(III) polypyridyl complexes with DNA
Abstract
This work investigates the interaction of rhodium polypyridyl complexes with DNA upon irradiation with UV light, particularly the complex, cis-dichlorobis(phenanthroline)rhodium(III). The exploration of the interactions of this complex with DNA was based on the study of a potential photo-chemotherapeutic methodology which combines light activation with the dark (non-light activated) covalent binding of a metal complex, such as the anti-cancer agent cisplatin (Pt(NH$\sb3$)$\sb2$Cl$\sb2$). We have also investigated the dark interactions of this complex, and analogs, with DNA. Based on this work we have determined that the complex stated above does covalently bind to DNA upon irradiation with long wavelength UV light. We observe minimal association in the absence of light. Variables such as the ionic strength of the reaction buffer, the presence of oxygen and the wavelength of irradiation have also been examined for their ability to effect covalent binding of the rhodium complex. We have also identified nucleoside-rhodium adducts formed upon photochemical reaction of the rhodium complex with DNA and nucleosides.
Degree
Ph.D.
Advisors
Morrison, Purdue University.
Subject Area
Organic chemistry
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