Application of the photothermal lens and development of second harmonic detection fluorometry in two-photon spectroscopy
Abstract
Continued progress is reported in the development of two-photon absorption instrumentation. Application of the thermal lens to two-photon absorption was made as a means to solve compromised utility of two-photon excited fluorescence due to poor fluorescence quantum yields. Instrument design, data collection, and results of these studies are presented. Improvements in two-photon excited fluorescence detectability are reported via second harmonic detection of sinusoidally modulated excitation. Instrument design, construction, and instrument performance analyses are discussed. Fluorometric detection limits are further improved via construction of a second generation instrument based on second harmonic detection, localization of the two-photon excitation, and improved emission collection efficiency.
Degree
Ph.D.
Advisors
Lytle, Purdue University.
Subject Area
Analytical chemistry
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