Interactions between the English grain aphid, Macrosiphum avenae (Homoptera:Aphididae) and isolates of the MAV serotype of barley yellow dwarf virus
Abstract
Symptomatic variants were subcultured from the NY-MAV (Rochow, 1984) isolate of the barley yellow dwarf virus (BYDV) by transmission with single Macrosiphum avenae allowed a 3-day infection feed on infected oats, then each allowed a one-hour infection feeding time on individual test plants. Infections were verified by ELISA. The symptoms expressed separated two distinct types of variants. Symptoms of one ('notch' subculture) included the production of notches, leaf twisting and development of an intense reddish color. Symptoms of the other ('red' subculture) included only the red coloration. These symptom-types were maintained through several successive transfers using groups of 5-10 aphids and longer feeding infection times. The experiments reported here show that variants differing markedly in symptoms can be subcultured from the NY-MAV isolate. Their serotypical properties and therein effects on the feeding behavior of M. avenae are also reported.
Degree
Ph.D.
Advisors
Foster, Purdue University.
Subject Area
Entomology|Plant pathology
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