Relation of vitamin B6 to growth and virus infection of feline tumor and nontumor cell lines
Abstract
The effects of different concentrations of vitamin B-6 (pyridoxine, PN-HCl) on the growth of feline lymphosarcoma (3201-R) and Crandell Feline Kidney (CRFK) cell lines and their feline leukemia virus (FeLV) infected counterparts (3201-R/FeLV and CRFK/FeLV) were studied over a five day period. Flasks were seeded with 5 x 10$\sp{5}$ live cells each and viable cell counts were made daily. The total numbers of 3201-R cells grown in media supplemented with 0, 0.02 and 0.25 mM PN-HCl were not significantly different; however, at 1.0 to 5.0 mM, 50 to 95% growth inhibition of tumor cells was observed in 3 to 5 days. Growth of CRFK cells did not differ significantly in medium supplemented with PN-HCl concentrations up to 1.0 mM whereas growth inhibition of 50 to 93% was observed at PN-HCl levels of 2.0 to 5.0 mM. Growth inhibition occurred more rapidly in virus-infected cells than uninfected cells. PLP represented 54 to 99% of the total vitamin B-6 present in extracts of cell grown in five different levels of PN-HCl. Intracellular PLP concentrations paralleled the levels of PN-HCl supplementation until a level was reached which inhibited cell growth. Concomitant with cell growth inhibition, intracellular PN increased significantly and PLP decreased. Susceptibility of CRFK cells to herpesvirus infection was examined in cells adapted to grow in high levels of PN-HCl. Cells grown in medium without additional PN-HCl were more easily infected with the virus than those grown in medium supplemented with 3.0 mM PN-HCl. The highest degree of infection was obtained in 1.0 mM PN-HCl which was also the upper limit of the range of PN-HCl supplements for maximal growth of CRFK cells. This investigation examined the metabolism of vitamin B-6 in feline lymphosarcoma and kidney cell lines, and the susceptibility to herpesvirus infection of non-tumor cells adapted to grow in high levels of PN-HCl. Concentrations of PN-HCl were identified which inhibited the growth of feline tumor cells and virus-infected tumor cells without deleterious effects on non-tumor cells. The findings indicate that vitamin B-6 is involved in regulating the growth of feline cell lines, and may be antineoplastic.
Degree
Ph.D.
Advisors
Burnstein, Purdue University.
Subject Area
Nutrition
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