Multistage mass spectrometry: Scan modes and new instrumentation
Abstract
A logical analysis of mass spectrometric scan modes is performed which reveals the full set of experiments available in multi-stage mass spectrometry. The analysis utilizes a symbolism and terminology which helps to provide an organizational scheme for representation and classification of the wide variety of experiments which exist. Data manipulation techniques which operate on data of different order and dimensionality to generate other forms of data are discussed and demonstrated. The analysis reveals several new types of scan modes including a last remaining MS/MS scan type referred to as the functional relationship scan, a last remaining MS/MS/MS scan having mass dimensionality of one referred to as the selective neutral-loss scan, and several MS/MS/MS scans which have two mass dimensions. Several new types of multi-stage mass spectrometers are described. Hybrid mass spectrometers are discussed in general and two examples are discussed in detail. A second generation hybrid BEqQ instrument is described along with the necessary electrical and computer interfacing required for its operation. The performance of the instrument for MS/MS/MS experiments is demonstrated using several examples of the various scans for obtaining sequence specific information about peptide samples. A detailed study of ion injection into a quadrupole ion trap operated in the mass instability mode resulted in the ability to construct a novel hybrid instrument. The instrument joins together a high resolution magnetic (B) and electric (E) sector mass spectrometer to a mass analyzing quadrupole ion trap (T) to form the first BET hybrid instrument. The advantages and performance of this instrument are described. The shortcomings of the hybrid BEqQ for performing some MS/MS/MS experiments are found to be overcome by an instrument which joins three mass analyzing and two rf-only quadrupole devices in tandem. This instrument is found to be quite suitable for implementing all the various MS/MS/MS scan modes using a relatively simple and inexpensive data system which is also described.
Degree
Ph.D.
Advisors
Cooks, Purdue University.
Subject Area
Analytical chemistry
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