Applied electric fields in the size-exclusion chromatography of proteins
Abstract
A method for separating proteins by a combination of size exclusion chromatography and electrophoresis has been demonstrated in our laboratory. The effects of heating, electroosmosis, and buffer polarization are found to be minimal. A first order mass balance is sufficient to describe the migration of small solutes in the column, but fails for solutes of comparable dimensions to the pore spaces in the gel. We have found that an electric field dependent adsorption term describes the deviation from the first order model well, for all the proteins and other macromolecules studied. These effects have been quantified, and may be used to predict enhancement of resolution in multicomponent samples. We have shown some separations as an example of the enhanced resolution available by this method.
Degree
Ph.D.
Advisors
Ladisch, Purdue University.
Subject Area
Chemical engineering
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