THE ROLE OF CAMPYLOBACTER SPUTORUM SUBSPECIES MUCOSALIS, CAMPYLOBACTER HYOINTESTINALIS AND CAMPYLOBACTER COLI IN THE ETIOLOGY OF PORCINE PROLIFERATIVE ENTERITIS
Abstract
The objective of this study was to further evaluate the role of Campylobacter sputorum subspecies mucosalis, Campylobacter hyointestinalis and Campylobacter coli infections in the etiology of porcine proliferative enteritis and to observe the effect of a non-pathogenic bacterium, Streptococcus faecium C-68, on a Campylobacter induced diarrhea. Neonatal gnotobiotic pigs and isolated loops of ileum from weanling conventional pigs were used. Localization, attachment and penetration by Campylobacter were evaluated by light and electron miroscopy. The bacterial growth at multiple levels of the intestine was quantitated. Isolates of Campylobacter sputorum subspecies mucosalis and Campylobacter hyointestinalis were tested separately and in combination. Combined Campylobacter sputorum subspecies mucosalis and Campylobacter hyointestinalis infections were also evaluated in an eight week sequential study. These studies showed that Campylobacter sputorum subspecies mucosalis and Campylobacter hyointestinalis could produce diarrhea and stimulate a mild inflammatory response in gnotobiotic pigs. Campylobacter sputorum subspecies mucosalis and Campylobacter hyointestinalis localized in the cecum and colon and showed an affinity for mucus and goblet cells. Bacteria were present near the surface of enterocytes but were not directly attached to cell membranes. Penetration of enterocytes was seen in the cecum of one animal at six weeks post exposure to a mixed infection of Campylobacter hyointestinalis and Campylobacter sputorum subspecies mucosalis. There was no intracellular bacterial colonization or proliferation of crypt epithelial cells observed.
Degree
Ph.D.
Subject Area
Veterinary services
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