TRANSPORT SYSTEMS AND ASSOCIATED DETECTORS FOR LIQUID CHROMATOGRAPHY
Abstract
Since the detection of polar pesticides utilizing previous technology is difficult, the thesis problem was to develop methodology or instrumentation applicable to the trace analysis of polar pesticides and their metabolites. Liquid chromatography is the method of choice for the separation of polar pesticides but its application to the problem has been limited by a lack of sensitive and specific detectors. As a solution to the problem, the development of a sensitive and element specific detector for liquid chromatography is described along with the details of construction and operation. In order to separate the organic solvents used in liquid chromatography from the solutes of interest, a transport system has been developed. Quartz fiber supported on a rotating disc has been selected as the conveyor material of the transport system. A stacked flame photometric detector (SFPD) is described which when used with the transport system can detect nanogram quantities of phosphorus- or sulfur-containing compounds with a wide dynamic range and adequate specificity. A flame ionization detector (FID) is described to prove the feasibility of its use with the transport system. The overall system is small and compact, yet rugged and is particularly well suited for the selective detection of phosphorus- and sulfur-containing compounds or for the detection of organic compounds in liquid chromatography. However, the system need not be limited to liquid chromatography.
Degree
Ph.D.
Subject Area
Analytical chemistry
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