USE OF A SILICON-INTENSIFIED TARGET VIDICON IN STUDIES OF SYNCHRONOUS FLUORESCENCE
Abstract
The thesis describes design and evaluation of a synchronous fluorimeter, which incorporates a silicon intensified target (SIT) vidicon and permits simultaneous acquisition of 100 emission spectra at each of 100 excitation wavelengths. These emission spectra appear in the form of a two-dimensional data matrix of intensities at different excitation wavelengths. Any desired value for a wavelength difference, (DELTA)(lamda), between the excitation and emission wavelengths can be obtained by extracting values from this matrix along parallels to the main diagonal of the matrix. Because there are many such values for (DELTA)(lamda) embedded in this matrix, one can be confident that the optimum value for the system under study is contained in the matrix. The instrument has been used to acquire emission and synchronous spectra for single- and two component samples of anthracene, 9,10-diphenylanthracene, perylene and tetracene. Relative pooled standard deviations on the order of 1% were obtained for the single component samples and on the order of 4-8% for the two-component samples. The glass envelope surrounding the detector has precluded observations in the ultraviolet, but the technique is easily extended to this region of the spectrum with another detector.
Degree
Ph.D.
Subject Area
Analytical chemistry
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