AFFINITY CHROMATOGRAPHY AND THE PLATE MODEL FOR NONLINEAR PACKED-COLUMN PROCESSES
Abstract
The packed-bed adsorption or chromatography theories are analyzed. The affinity chromatography process is reviewed. An extended plate model is postulated for the analysis of nonlinear chromatography systems. The nonlinear-system plate height expression is developed by extending the linear-system HETP. An interpretation of the plate height is given to identify the problem. The nonlinearity of the adsorption isotherm is accounted for through a continuous adjustment of the plate height. The non-equilibrium behavior of the process is considered. The model is tested by comparing its results with the rigorous rate theory predictions. The plate concept in modeling the chromatographic process and its model developing strategy are utilized to analyze the nonlinear packed-column heterogeneous catalysis. A plate height expression is derived and its applications are discussed. The mathematical treatments of quantitative affinity chromatography are presented. A general expression for the retention volume is derived, which includes effects of the frequently occurring nonspecific adsorption interferences, as well as the biospecific interactions. Several cases are discussed. The gradient elution of quantitative affinity chromatography is analyzed. A relation for the eluent concentration and various parameters is derived.
Degree
Ph.D.
Subject Area
Chemical engineering
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