Studies of the Newcastle Disease Virus in Tissue Culture
Abstract
The constant threat of Newcastle disease (ND) to the poultry industry indicates a continued need for basic research leading to improved methods of its diagnosis and a better understanding of its epizootiology.The first objective of this study was to establish a practical method of propagating Newcastle disease virus (NDV) in tissue cultures of mammalian cells. Earlier studies indicated that this virus survived for brief periods on swine embryo kidney cells in vitro. A "line" of swine embryo lymph node cells was maintained over a two year period. All cells used in this study were maintained in Eagle's tissue culture medium with added antibiotics, mycostatin, glutamine, and varied concentrations of swine, chicken, or human serum.The virus grew in these cells and had a marked cytopathic effect upon them that was inhibited with NID immune serum. Virus diluted to the end point of its infectivity for chicken embryos (10-9) initiated infection in the swine cells. The high concentrations of virus (107 CELD50's per 0.1 ml. of tissue culture supernatant fluid) obtained with regularity indicated that this is a practical, economical method for propagating NIV in large quantities in a system free from avian pathogens that might be found in the chicken embryo.
Degree
Ph.D.
Subject Area
Animal sciences
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