AN INSTRUMENTAL METHOD FOR MEASURING FLUORESCENCE AND A STUDY OF FLUORESCENT RUTHENIUM POLYAMINE COMPLEXES
Abstract
The Perkin-Elmer Spectracord was successfully adapted for use as a recording spectrofluorescimeter. Fluorescing systems were activated with monochromatic energy from a 6000 watt xenon arc, and the fluorescence was analyzed utilizing the optics of the Spectrecord. A method for automatically recording activation spectra for strongly fluorescing systems was developed. The effect of spectral response of the detector on the wavelength maxima was determined and in the region of 500 to 600 mp a semiquantitative correction of +1 to +5 mp was applied. The electronic gain of the instrument proved to be an important factor in attaining reproducibility. Evidence was obtained to show that the intensity of the xenon are was stable over prolonged time periods. The performance of the instrument as a spectrofluorescimeter was shown to be satisfactory by comparison to published
Degree
Ph.D.
Subject Area
Inorganic chemistry|Chemistry
Off-Campus Purdue Users:
To access this dissertation, please log in to our
proxy server.