Glycine soja into Glycine max to increase its genetic base
Abstract
Soybean production in the US contends with several invasive pests, but few compare to the soybean aphid that migrates to soybean fields during the summer growing season and feeds on pod, stem, and leaf tissue. The soybean aphid is green-bodied and reproduces asexually causing rapid population explosion. Its ability to impose major yield loss has driven breeding efforts. Five genes controlling a form of resistance to this insect were identified in the last seven years, but biotype adaptation has decreased the lifespan of some of these insect resistance genes. It would be beneficial to have more durable resistance such as is found in the wild relatives of soybean. Glycine soja, the wild progenitor of Glycine max, is one such source. There are about 2,600 G. soja accessions available in the GRIN seed bank. Next-generation sequencing technology (NGS) has made genetic mapping of natural variation cost effective, especially for wild relatives that are resource-poor. In this thesis research, I applied genotype-by-sequencing (GBS) to pooled populations to study the inheritance of genomic regions in G. soja segregating for resistance to the soybean aphid. Two F2 cross populations were created from accessions PI 549046 and PI 483463 and used for the genotyping analysis. Here I report regions on chromosomes 1, 6, and 7 in PI 483463 and a region on chromosome 16 in PI 549046 that are segregating for resistance to the soybean aphid.
Degree
Ph.D.
Advisors
Ma, Purdue University.
Subject Area
Agronomy|Plant sciences
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