Instrumentation and method development for the interrogation of biomolecules and ion/ion chemistry
Abstract
Instrumentation and new methods are investigated for their utility in biomolecule analysis as well as the investigation of new ion/ion chemistry. The instrumental emphasis for these investigations have utilized 2D linear ion traps for their improvement in sensitivity, dynamic range, and the ability to mate these analyzers with other pre-existing mass spectrometry requirements. Other instrumental emphasis is placed on resolution and mass accuracy, which is effected by the time-of-flight mass analysis. Developing and implementing methods on these instruments provide a diverse array of approaches in order to solve current analytical problems within the context of biomolecular analysis. Development of a 2D linear ion trap for high-mass biomolecule analysis is discussed for implementing a large range of interrogation techniques. Multiple configurations and ionization sources are available and interchangeable on this highly flexible platform. These methods are discussed primarily within the context of a single quadrupole linear ion trap, along with expansion into configurations including two linear ion traps arranged in nonlinear fashion. Instrumentation for low-mass biomolecules originating from vegetation is also discussed. An instrument is modified to support investigations of monoterpenes in ambient atmospheric concentrations is described. Biomolecule analysis data have been used to identify proteins and peptides to support the proteomics science. Methods and experimental techniques are described to improve the results obtained in bioinformatics and mass spectrometry. Finally, characterization of new ion/ion chemistry in the form of electron/peptide interaction is described. Electron transfer dissociation (ETD) is discussed in the context of charge identity and position within the peptide. A description of product partitioning is formed for the channels of dissociative reaction resulting in backbone and neutral loss as well as nondissociative reaction and proton transfer.
Degree
Ph.D.
Advisors
McLuckey, Purdue University.
Subject Area
Analytical chemistry
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