Protein microencapsulation using ultrasonic atomizer: Process optimization

Mingli Ye, Purdue Libraries

Abstract

Protein microencapsulation into polymeric metrics is generally considered as an effective way to improve patient compliance when the encapsulated protein drug releases in a sustained manner. In this study, the process and formulation parameters of lysozyme microencapsulation by ultrasonic atomizer was optimized, followed by the characterization of encapsulated lysozyme. The microcapsules prepared under optimized condition had a mean size of 65.3±3.4 μm, lysozyme encapsulation efficiency of 77.6±7.3% and a moderate lysozyme loading of 4.55±0.43%. These microcapsules had smooth surfaces and limited surface defects. The in vitro release of lysozyme in a 35-day period showed a continuous release to 50.9% of encapsulated lysozyme with a moderate high burst release of 25.9%. The released lysozyme kept its integrity and activity. In order to further decrease lysozyme burst release, the cryoprotectants, such as sugars, were screened as the freeze drying media. The burst release of these microcapsules was reduced but a slowed-down release profile in a 14-days period was also observed. Finally, several polymer-organic solvent systems were screened as the candidates of protein microencapsulation. Polystyrene-chloroform system showed the best ability to encapsulate aqueous solution.

Degree

Ph.D.

Advisors

Park, Purdue Libraries.

COinS