Investigating the role of ankyrin repeat and SOCS box containing protein (ASB) 15 in skeletal muscle
Abstract
The purpose of the present study was to investigate the role of ASB15 in skeletal muscle growth. Ankyrin repeat and SOCS box protein (ASB) 15 belongs to a family of genes characterized by the presence of both an ankyrin repeat and SOCS (suppressor of cytokine signaling) box motif. In the present study, we evaluate ASB15 through sequence analysis and animal and cell culture models. The complete bovine ASB15 coding sequence is 1766 by in size and contains multiple ankyrin repeat motifs and a single SOCS box motif. Using rodent models, we report that Asb15 mRNA levels decrease after administration of the β-AR agonist clenbuterol. Ectopic expression of an ASB15 expression construct also increases skeletal muscle fiber cross section area when compared to control animals ectopically expressing a control pcDNA3.1 expression construct. Through cell culture models, we report that Asb15 mRNA levels increase significantly upon differentiation of C2C12 myoblasts. Stable C2C12 cell lines expressing the full length ASB15 also have decreased creatine kinase levels and protein synthesis on day 1 of differentiation suggesting a delay in differentiation. On day 3 of differentiation, protein synthesis increases for the ASB15-FL line and protein degradation in greater for the ASB15-Ank line. This change in protein synthesis and degradation could affect protein turnover and fiber size in vivo as reported previously for ectopic expression of ASB15. This research provides a base for the possible function of ASB15 in skeletal muscle development.
Degree
Ph.D.
Advisors
Moody, Purdue University.
Subject Area
Molecular biology
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