Materials analysis using chemical imaging
Abstract
This thesis describes various applications of chemical analysis using Near-Infrared Raman Imaging Microscopy (NIRIM) and fluorescent optical imaging microscopy. The NIRIM instrument used for these studies combines fiber-bundle image compression (FIC) hardware and multivariate signal processing software to identify and map the distribution of chemical species on a sample surface. Applications include mapping the distribution of elemental sulfur on oxidized pyrite and arsenopyrite surfaces, the distribution of chemical species such as thaumasite, ettringite, and gypsum in cement samples produced with and without exposure to sulfate, the distribution of both iron oxides and oxyhydroxides on naturally occurring iron minerals and corroded steel surfaces, and finally the distribution of solid lubricants on tribological surfaces, both inside and outside the wear tracks created under different load and lubrication conditions. In addition, optical fluorescent imaging microscopy combined with selective chemical targeting is used to obtain enhanced images of biological tissues. More specifically, a new in-vivo optical imaging system has been developed for detecting folate-receptor positive tissues in mice using folate-linked fluorescein dye conjugates. Tumor bearing mice were injected with folate fluorescein and the uptake of the fluorescent compound was found to localize in tumorous tissues as well as in normal tissues that contain high levels of folate receptor such as kidneys, choroid plexus, and a xenograft of an M109 lung epithelial tumor cell line.
Degree
Ph.D.
Advisors
Ben-Amotz, Purdue University.
Subject Area
Analytical chemistry|Civil engineering|Oncology
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