Designing Stationary Phases for Improved Protein Separations
Abstract
As monoclonal antibodies (mAbs) become a more important part of the pharmaceutical industry, the need for better quicker analysis of then will also increase. To do this, better stationary phases specifically designed for mAbs need to be developed to analyze the quality of mAbs by their critical control attributes. The three main critical control attributes are size, charge, and glycosylation. This work focuses mainly on the development of stationary phases to analyze critical control attributes in size and charge through using a non-porous silica base and surface confined atom transfer radical polymerization to grow improved stationary phases that minimize undesired interactions and maximize desired interactions.
Degree
Ph.D.
Advisors
Wirth, Purdue University.
Subject Area
Analytical chemistry|Chemistry|Electromagnetics|Fluid mechanics|Mechanics|Medicine|Pharmaceutical sciences|Physics
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