Analysis of vitamin e metabolites by liquid chromatography-tandem mass spectrometry
Abstract
Naturally occurring forms of Vitamin E are metabolized to various carboxychromanols and conjugated carboxychromanols. Recent studies showed that vitamin E metabolites, especially the long-chain carboxychromanols are more bioactive than unmetabolized vitamin E forms. It is necessary to quantify vitamin E metabolites in biological environment. Here a simple and effective extraction method was developed to achieve extraction efficacy of more than 90% of various forms of vitamin E and metabolites with less than 10% inter- or intra-day variation. An LC-MS/MS assay was developed and optimized to acquire best sensitivity for the detection of vitamin E and metabolites. This method allows simultaneous detection of all carboxychromanols and sulfated carboxychromanols. Using the optimized extraction and LC-MS/MS analysis conditions, vitamin E metabolites in plasma or feces of animals fed with γT, γTE, δT or δTE-13' were analyzed. Results showed that, the major metabolites in the blood were conjugated g-CEHC and sulfated long chain carboxychromanols.
Degree
M.S.
Advisors
Jiang, Purdue University.
Subject Area
Nutrition
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