A fluorescence correlation spectroscopy study of the diffusion of macromolecules in inhomogeneous biological media
Abstract
Diffusion of particles in inhomogeneous media is not yet well understood. Diffusion coefficients were experimentally measured for a variety of dextran sizes in solution, agarose gel, and porcine corneal stroma using Fluorescence Correlation Spectroscopy. The hydrodynamic radii of the dextrans are determined from their diffusion coefficients in solution. The measured diffusion coefficients in media are compared with the current theory, past works, and theoretical models. Diffusion coefficients in agarose gel and cornea are found to have a greater dependence on hydrodynamic radius than is predicted by the Stokes-Einstein relation.
Degree
M.S.
Advisors
Todd, Purdue University.
Subject Area
Physics|Biophysics
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