The effects of phytate and phytase on phosphorus homeostasis and active phosphate transport in the small intestine of rats with chronic kidney disease
Abstract
The purpose of the experiments in chapters 2 and 3 was to investigate the effects of phytate and phytase on phosphorus homeostasis and active phosphate transport in the small intestine of rats with chronic kidney disease. Phosphorus and calcium concentrations in serum, urine and stool were measured. Creatinine concentrations in plasma and urine, and intact PTH concentrations were also measured to evaluate the progression of chronic kidney disease. Active phosphate transport in the jejunum was determined by Ussing chambers. NaPi-IIb gene expression was measured in proximal duodenum, distal duodenum and jejunum. The study in chapter 2 was conducted to determine the effect of phytate on phosphorus homeostasis and active transport in the small intestine of rats with chronic kidney disease. The results demonstrated that phytate did not affect serum phosphorus and plasma creatinine concentrations, but decreased urine and stool phosphorus concentrations. No changes were observed in active phosphate transport in the jejunum. Phytate did not affect NaPi-IIb gene expression in the proximal and distal duodenum, but interestingly, decreased its expression in the jejunum. The second study in chapter 3 was designed to determine the effect of phytase on phosphorus homeostasis and active transport in the small intestine of rats with chronic kidney disease. The results indicated that phytase did not affect phosphorus homeostasis and active phosphate transport in the jejunum. NaPi-IIb gene expression was also not affected. Adding phytase did not aggravate the progression of chronic kidney disease.
Degree
M.S.
Advisors
Radcliffe, Purdue University.
Subject Area
Animal sciences|Animal Diseases
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