THE EFFECT OF CERTAIN CARCINOGENIC AGENTS ON THE TOTAL NON-PROTEIN SULFHYDRYL CONTENT OF THE RAT LIVER
Abstract
These experiments indicate that fasting alone (20-24 hours) had very little effect on the TNPSH content of the rat liver. From acute experiments, it was found that DAB, administered up to 50 mg./Kg., failed to produce any significant decrease in the sulfhydryl level, whereas BNA, given at a dose of either 10 mg. or 20 mg./ Kg., induced a significant and similar drop in the TNPSH concentration. A dose of 5 mg./Kg. of BNA was without any effect.The results, from the chronic experiments, revealed that the TNPSH level of the stock diet fed rats remained relatively constant throughout the experiment. The low protein-low riboflavin diet fed rats showed a decrease after 12 weeks but the liver TNFSH values returned to near normal in 16 weeks. There is also indication that the addition of 600 mg. of DAE or 100 mg. of BNA per 1 Kg. of diet decreased the sul- fhydryl concentration of the rat liver. The incorporation of BNA to a non-protective diet caused a maximum drop after 4 weeks. Under the same experimental conditions, the effect of DAB was quite marked after 8 weeks, with very little variations at the end of 16 weeks.
Degree
Ph.D.
Subject Area
Pharmacology
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