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NCES Discipline

Biochemistry and Molecular Biology

Sub-discipline

Histone Protein Modification

Abstract

The researcher is conducting research on the brain cells of drosophila larvae. Specifically, the goal is to sort nuclei from glial and neuronal cells from the brains of mutant and wild type drosophila larvae. She will do transcriptome and high throughput ChIP-seq analysis on the cell nuclei to examine the distribution of complexes that modify histones and how loss of those complexes affects transcription. The stages of her research involve a long period of methodology development, a shorter data collection period, and then data analysis. The lab notebook contains written documentation of all experiments and trials and links to data, versions of analysis and images, databases, and versions of the developing journal article. Therefore, keeping an organized electronic lab notebook is of high importance for the researcher. It is also very important for her students to be trained to properly document their experiments using her lab notebook structures and protocols. The researcher submits some of her data to a database or repository as a part of the publication process. This data is publicly available once the article is published. The researcher has no limitations on access to the data, except that she requires that those using or referencing her data cite the paper from which it came rather than the data itself.

DOI

10.7771/2326-6651.1034

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