Effect of Oxygen Concentration on the Formation of Malondialdehyde-Like Material in a Model of Tissue Ischemia and Reoxygenation
This study was conducted to explore the functional relationship between oxygen concentration during tissue reoxygenation after ischemia and the extent of postischemic lipid peroxidation, an indicator of reoxygenation injury. Excised rat liver or kidney tissue was rendered ischemic for 1 h at 37°C, minced into 1 mm3 fragments, and then reoxygenated for 1 h in flasks of buffered salt solution containing various amounts of oxygen. Production of malondialdehyde-like material (MDA) was measured to indicate lipid peroxidation. MDA production was minimal at oxygen tensions less than 10 mmHg, increased sharply from 10 to 50 mmHg, and plateaued at approximately 100 mmHg. A similar functional relationship was produced by a simple mathematical model of free radical mediated lipid peroxidation in biological membranes, suggesting that MDA production is indeed caused by free radical oxidation of membrane phospholipids and that the oxygen effect is governed by simple competition between chain propagation and chain termination reactions within the membrane. These experimental and analytical results confirm that relatively low concentrations of oxygen arc sufficient to produce oxidative damage in post-ischemic tissues.
Date of this Version
Salaris, Steven C. and Babbs, Charles F., "Effect of Oxygen Concentration on the Formation of Malondialdehyde-Like Material in a Model of Tissue Ischemia and Reoxygenation" (1989). Weldon School of Biomedical Engineering Faculty Publications. Paper 86.
This is the author accepted manuscript of Salaris S.C., and Babbs, C.F., Effect of oxygen concentration on the formation of malondialdehyde-like material in a model of tissue ischemia and reoxygenation, Free Radical Biology & Medicine 7, 603-609, 1989. Copyright Elsevier, it is made available here CC-BY-NC-ND, and the version of record can be found at http://dx.doi.org/10.1016/0891-5849(89)90141-X.