Keywords
Lactate biosensor, biosensor, oxygen storage capacity, ceria biosensor, amperometric biosensor
Presentation Type
Event
Research Abstract
Amperometric lactate biosensors are used to detect lactate concentration in blood and tissues, which is integral in identifying cyanide poisoning, septic shock, and athletic condition. The construction of lactate biosensors with high sensitivity, selectivity, and stability is imperative to diagnose and determine these medical conditions. Lactate detection is currently limited to oxygen-rich environments due to the fact that oxygen is a limiting factor in the lactate reaction. To circumvent this problem, researchers have developed mediators or alternate, oxygen-free enzymes to improve sensitivity. In our study, ceria (CeO2) with high oxygen storage capacity (OSC) was introduced to the enzyme layer to eliminate the effects of oxygen depletion. Fluctuation in oxygen concentration was combatted by use of ceria metal oxide nanopowders, which absorb and release oxygen under oxygen rich and lean conditions respectively. These nanopowders were deposited on the electrode surface in a polyelectrolyte solution. The lactate biosensors were then constructed using layer-by-layer assembly to take advantage of electrostatic interaction between the positively charged polyelectrolyte and negatively charged lactate oxidase (LOx). Polyethylenimine (PEI), a positively charged polymer, was used to immobilize the enzymes on the Pt surface via alternating electrostatic adsorption. It was observed that the introduction of ceria in the enzyme layer reduced oxygen dependency. The results showed that lactate biosensors with high selectivity, sensitivity, and wide detection limit were constructed.
Session Track
Sensing
Recommended Citation
Elizabeth Andreasen, Lia Stanciu, and Aytekin Uzunoglu,
"The Role of Metal Oxide Layers in the Sensitivity of Lactate Biosensors Subjected to Oxygen-Limited Conditions"
(August 7, 2014).
The Summer Undergraduate Research Fellowship (SURF) Symposium.
Paper 126.
https://docs.lib.purdue.edu/surf/2014/presentations/126
The Role of Metal Oxide Layers in the Sensitivity of Lactate Biosensors Subjected to Oxygen-Limited Conditions
Amperometric lactate biosensors are used to detect lactate concentration in blood and tissues, which is integral in identifying cyanide poisoning, septic shock, and athletic condition. The construction of lactate biosensors with high sensitivity, selectivity, and stability is imperative to diagnose and determine these medical conditions. Lactate detection is currently limited to oxygen-rich environments due to the fact that oxygen is a limiting factor in the lactate reaction. To circumvent this problem, researchers have developed mediators or alternate, oxygen-free enzymes to improve sensitivity. In our study, ceria (CeO2) with high oxygen storage capacity (OSC) was introduced to the enzyme layer to eliminate the effects of oxygen depletion. Fluctuation in oxygen concentration was combatted by use of ceria metal oxide nanopowders, which absorb and release oxygen under oxygen rich and lean conditions respectively. These nanopowders were deposited on the electrode surface in a polyelectrolyte solution. The lactate biosensors were then constructed using layer-by-layer assembly to take advantage of electrostatic interaction between the positively charged polyelectrolyte and negatively charged lactate oxidase (LOx). Polyethylenimine (PEI), a positively charged polymer, was used to immobilize the enzymes on the Pt surface via alternating electrostatic adsorption. It was observed that the introduction of ceria in the enzyme layer reduced oxygen dependency. The results showed that lactate biosensors with high selectivity, sensitivity, and wide detection limit were constructed.